MEM (Minimum Essential Medium) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of MEM modifications for a range of cell culture applications.
This MEM is modified as follows:
With |
Without |
• NEAA |
• HEPES |
• L-glutamine |
|
• Phenol Red |
|
D-Glucose (Dextrose)(1000mg/L) |
|
保存条件
2-8° C
运输条件:
常温
使用方法:
MEM was developed by Harry Eagle, and was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks' salts for use without CO2. This product is made with Earle’s salts. MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
注意事项
在使用的过程中要特别注意避免溶液被微生物与酶污染。
为了您的安全和健康,请穿实验服并戴一次性手套操作。
备注:所有报价均已含国内快递费及发票费用。库存情况可能有所变动,请与我们销售人员确认为准,价格情况请直接联系我们销售人员。