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Dulbecco`s Modifified Eagle Me

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Dulbecco`s Modifified Eagle Me

DMEM high glucose no glutamine no calcium

DMEM (Dulbeccos Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts neurons glial cells HUVECs and smooth musc...

Product Description

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications.

This DMEM is modified as follows:

With Without
• High Glucose • L-glutamine
• Phenol Red • Sodium Pyruvate
  • Calcium
  • HEPES
   
 

The complete formulation is available.

Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Product use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.
specifications
Form: Liquid
Glucose: High Glucose
Glucose Concentration: 4500 mg/L
Glutamine: No Glutamine
HEPES Buffer: No HEPES
Phenol Red Indicator: Phenol Red
Product Line: VrctoryX
Product Size: 500 mL
Shipping Condition: Room Temperature
Sodium Pyruvate Additive: No Sodium Pyruvate
Storage： 2℃～8℃
Volume (Metric): 500 ml

Pre：DMEM High Glucose no Glutamine no methionine no cystine
Next：DMEM High Glucose HEPES

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