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Minimun Essential Media

MEM α no Nucleosides

MEM (Minimum Essential Medium ) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells. MEM can be used with a variety of suspension and adherent mammalian cells including keratinocytes primary...

Product Description

MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of MEM α modifications for a range of cell culture applications.

This MEM α is modified as follows:


With Without
• Phenol Red • Ribonucleosides
• L-glutamine • Deoxyribonucleosides

The complete formulation is available.

Using DMEM α
使用DMEMα
MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR-negative cells. This product is made with Earle’s salts. MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% 
CO environment to maintain physiological pH.


For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.


Form: Liquid
Glucose: Low Glucose
Glutamine: L-Glutamine
HEPES Buffer: No HEPES
Phenol Red Indicator: Phenol Red
Product Line: VictoryX
Product Size: 500 mL
Shipping Condition: Room Temperature
Sodium Pyruvate Additive: Sodium Pyruvate
Volume (Metric): 500 ml

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