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Liebovitz`s L15 Media

Ham’s F-12 Nutrient Mix

Hams F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian...

Product Description

Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. We offer a variety of F-12 modifications for a range of cell culture applications.

This Ham's F-12 is modified as follows:


With
• L-glutamine
• Phenol Red

The complete formulation is available.

Using F-12

Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L), and therefore requires a 5–10% 
COenvironment to maintain physiological pH.

For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.


Form: Liquid
Glutamine: L-Glutamine
HEPES Buffer: No HEPES
Phenol Red Indicator: Phenol Red
Product Line: VictoryX
Shipping Condition: Room Temperature
Sodium Pyruvate Additive: Sodium Pyruvate
Volume (Metric): 500 ml

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