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Liebovitz`s L15 Media

M199

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology vaccine production a...
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Product Description

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium and rat lens tissues. We offer a variety of Medium 199 modifications for a range of cell culture applications.

This M199 is modified as follows:

With Without
• Phenol Red • HEPES
• L-glutamine  
 
The complete formulation is available.

Using Medium 199

Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a 
CO2 incubator, or with Hanks' salts for use withouCO2 This product contains Earle's salts. Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% COenvironment to maintain physiological pH.

For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.


Form: Liquid
Glucose: Low Glucose
Glutamine: L-Glutamine
HEPES Buffer: No HEPES
Phenol Red Indicator: Phenol Red
Product Line: VictoryX
Product Size: 500 mL
Shipping Condition: Room Temperature
Sodium Pyruvate Additive: No Sodium Pyruvate
Volume (Metric): 500 ml

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